13色流式細胞儀_Beckman CytoFLEX Flow Cytometer

放置地點
慈濟大學中央校區勤耕樓 D603-6  流式細胞儀分析實驗室

儀器介紹
流式細胞儀為分析光散射信號和螢光信號，利用光訊號的強弱和波長不同，能夠得知細胞大小、型態與顆粒化，提供多種細胞生物學特性，以應用於細胞實驗之中。CytoFLEX Flow Cytometer 將光學部件和流動室進行整合， 使光在傳輸中的損失降到最低，從而帶來最佳的儀器靈敏度。本儀器最高可以使用十三色雷射光譜，相容各類螢光染劑搭配，可自由運用搭配。可靠的採樣和流體系統設計支援微量上樣管的樣本獲取， 樣本上樣體積最小達到10 ul。CytoFLEX在多參數應用中同樣展現出強大實力，對微顆粒的檢測極限可低至200 nm。流式細胞儀最主要的實驗分析有細胞週期分析、細胞凋亡、細胞型態、細胞內pH值測定、細胞內鈣離子濃度測定，可應用於腫瘤學、免疫學、血液學和藥理學之應用研究。

 

A Flow Cytometer analyzes light scatter and fluorescent signals. By utilizing the differing intensities and wavelengths of these optical signals, it can determine various cell biological characteristics such as cell size, morphology, and granularity, which are applied in cell experiments.

The CytoFLEX Flow Cytometer integrates optical components and the flow cell to minimize light transmission loss, thereby delivering optimal instrument sensitivity.

This instrument can use up to 13-color laser spectrums, compatible with various fluorescent dyes for flexible configuration. Its reliable sampling and fluidic system design supports micro-volume sample acquisition, with a minimum sample volume of 10 µl.

The CytoFLEX also demonstrates strong capability in multi-parameter applications, with a detection limit for microparticles as low as 200 nm.

The main experimental analyses performed by a flow cytometer include cell cycle analysis, apoptosis, cell morphology, intracellular pH measurement, and intracellular calcium ion concentration measurement. It is applicable in research areas such as oncology, immunology, hematology, and pharmacology.

成果發表

  1. Jaceosidin, a natural polymethoxyflavone, suppresses leukemia cell growth and impairs lipid metabolism-dependent survival in AML and cytarabine-resistant cells
    Trung Q. Phan, Je-Wen Liou, Liang-In Lin, Wei-Han Huang, Ming-Jiuan Wu, Pei-Yi Chen, Jui-Hung Yen
    Biomed Pharmacother. 2025 Nov:192:118654. doi: 10.1016/j.biopha.2025.118654.
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  2. Therapeutic Potential of Salvia miltiorrhiza Root Extract in Alleviating Cold-Induced Immunosuppression
    Chi-Cheng Li ,Song-Lin Liu ,Te-Sheng Lien ,Der-Shan Sun ,Ching-Feng Cheng ,Hussana Hamid ,Hao-Ping Chen ,Tsung-Jung Ho ,I-Hsin Lin ,Wen-Sheng Wu ,Chi-Tan Hu ,Kuo-Wang Tsai and Hsin-Hou Chang
    Int. J. Mol. Sci. 2024, 25(17), 9432;https://doi.org/10.3390/ijms25179432
    -
  3. Targeted Delivery to Dying Cells Through P-Selectin–PSGL-1 Axis: A Promising Strategy for Enhanced Drug Efficacy in Liver Injury Models
    Te-Sheng Lien ,Der-Shan Sun and Hsin-Hou Chang
    Cells 2024, 13(21), 1778;https://doi.org/10.3390/cells13211778
    -
  4. Dengue Envelope Protein as a Cytotoxic Factor Inducing Hemorrhage and Endothelial Cell Death in Mice
    Te-Sheng Lien ,Der-Shan Sun ,Wen-Sheng Wu and Hsin-Hou Chang
    Int. J. Mol. Sci. 2024, 25(19), 10858;https://doi.org/10.3390/ijms251910858
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  5. Restraint Stress-Induced Neutrophil Inflammation Contributes to Concurrent Gastrointestinal Injury in Mice
    Rina Munalisa ,Te-Sheng Lien ,Ping-Yeh Tsai ,Der-Shan Sun ,Ching-Feng Cheng ,Wen-Sheng Wu ,Chi-Cheng Li ,Chi-Tan Hu ,Kuo-Wang Tsai ,Yungling Leo Lee ,Yu-Chi Chou and Hsin-Hou Chang
    Int. J. Mol. Sci. 2024, 25(10), 5261;https://doi.org/10.3390/ijms25105261
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