倒立共軛焦顯微鏡(含活細胞培養系統)_Nikon A1+

儀器介紹

Groundbreaking FOV開創性超大掃瞄視野數；採用25mm field of view掃瞄視野數，相較於只有FOV 18mm的傳統共軛焦顯微鏡，提供約2倍大的大尺寸影像。
大圖拼接更快速，當配置電動XY載物台時，25mm超大拍照視野數，可顯著減少拼接次數與拍照次數，並降低螢光漂白(Photo Bleaching)對分子結構的破壞。
高速高解析共振式掃瞄器resonant scanner共振式掃瞄器解析度提升至1024 x 1024，影像品質再升級。
Resonant scanner 的高速掃瞄速度，可降低活細胞光毒性傷害。

Groundbreaking FOV (Field of View) Ultra-Large Scanning Area: Adopts a 25 mm scanning field of view, providing an image size approximately two times larger compared to conventional confocal microscopes with only an 18 mm FOV.
Faster Large-Area Tiling: When configured with a motorized XY stage, the ultra-large 25 mm imaging FOV significantly reduces the number of stitching and acquisition steps, and minimizes photobleaching damage to molecular structures.

High-Speed, High-Resolution Resonant Scanner: The resonant scanner resolution is upgraded to 1024×1024, further improving image quality.

The high scanning speed of the resonant scanner can reduce phototoxicity damage in live cells.

Instrument Specifications
• Objectives: 10X, 20X, 40X, 60X (Oil), 100X (Oil)
• Lasers: 405 nm, 488 nm, 561 nm, 640 nm
• Live Cell Culture System

成果發表

Zebrafish PRL-3 Regulates Yolk Syncytial Layer Integrity and Actomyosin Contractility During Epiboly
Ting-Fang Wang, Kai-Wen Cheng, Yau-Hung Chen and Ming-Der Ling
Int. J. Mol. Sci. 2026, 27(5), 2339; https://doi.org/10.3390/ijms27052339
Developmental expression of Ap-Vas proteins in aphids and flies reveals their evolutionary roles in insects
Gee-Way Lin, Szu-Chieh Wang, Charles E Cook, Ming-Der Lin, Chun-Che Chang
Insect Mol Biol. 2025 Nov 30.doi: 10.1111/imb.70020.
Dengue Envelope Protein as a Cytotoxic Factor Inducing Hemorrhage and Endothelial Cell Death in Mice
Te-Sheng Lien ,Der-Shan Sun ,Wen-Sheng Wu and Hsin-Hou Chang
Int. J. Mol. Sci. 2024, 25(19), 10858;https://doi.org/10.3390/ijms251910858
Saussurea involucrata (Snow Lotus) ICE1 and ICE2 Orthologues Involved in Regulating Cold Stress Tolerance in Transgenic Arabidopsis
Chia-Ling Wu, Lee-Fong Lin, Hsiao-Chun Hsu, Li-Fen Huang, Chung-Der Hsiao and Ming-Lun Chou
IJMS; 2021/ Oct 7/ 22(19):10850. doi: 10.3390/ijms221910850.
Dissecting the role of a plant-specific Rab5 small GTPase NbRabF1 in Bamboo mosaic virus infection.
Ying-Ping Huang, Pei-Yu Hou, I-Hsuan Chen, Yau-Huei Hsu, Ching-Hsiu Tsai, Chi-Ping Cheng
Journal of Experimental Botany; 2020 Dec 31;71(22):6932-6944. doi: 10.1093/jxb/eraa422
Drosophila decapping protein 2 modulates the formation of cortical F-actin for germ plasm assembly
Yi-Mei Lee, Po-Hsun Chiang, Jen-Ho Cheng, Wei-Hong Shen, Chao-Han Chen, Mei-Ling Wu, Yi-Lu Tian, Chao-Heng Ni, Ting-Fang Wang, Ming-Der Lin, Tze-Bin Chou
Dev Biol ; 2020 May 1;461(1):96-106. doi: 10.1016/j.ydbio.2020.01.013
Oogenesis of Hematophagous Midge Forcipomyia taiwana (Diptera: Ceratopogonidae) and Nuage Localization of Vasa in Germline Cells
Szu-Chieh Wang, Yung-Hao Ching, Preethi Krishnaraj, Guan-Yu Chen, Anna Shiny Radhakrishnan, Hsien-Min Lee, Wu-Chun Tu, Ming-Der Lin
Insects ; 2020 Feb 5;11(2):106. doi: 10.3390/insects11020106
In vivo pro-angiogenic effects of dracorhodin perchlorate in zebrafish embryos: A novel bioactivity evaluation platform for commercial dragon blood samples
Preethi Krishnaraj, Yu Chang, Tsung-Jung Ho, Nai-Chen Lu, Ming-Der Lin, Hao-Ping Chen
journal of food and drug analysis ; 2019 Jan;27(1):259-265. doi: 10.1016/j.jfda.2018.08.005