{"id":6047,"date":"2020-10-07T11:37:41","date_gmt":"2020-10-07T03:37:41","guid":{"rendered":"http:\/\/research.tcu.edu.tw\/?page_id=6047"},"modified":"2026-06-04T08:10:17","modified_gmt":"2026-06-04T00:10:17","slug":"13%e8%89%b2%e6%b5%81%e5%bc%8f%e7%b4%b0%e8%83%9e%e5%84%80_beckman-cytoflex-flow-cytometer","status":"publish","type":"page","link":"https:\/\/research.tcu.edu.tw\/?page_id=6047","title":{"rendered":"13\u8272\u6d41\u5f0f\u7d30\u80de\u5100_Beckman CytoFLEX Flow Cytometer"},"content":{"rendered":"<div id=\"pl-6047\"  class=\"panel-layout\" ><div id=\"pg-6047-0\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-6047-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-6047-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<p><a href=\"https:\/\/research.tcu.edu.tw\/wp-content\/uploads\/2020\/12\/13_\u260513\u8272\u6d41\u5f0f\u7d30\u80de\u5100_Beckman-CytoFLEX-Flow-Cytometer.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-6523\" src=\"https:\/\/research.tcu.edu.tw\/wp-content\/uploads\/2020\/12\/13_\u260513\u8272\u6d41\u5f0f\u7d30\u80de\u5100_Beckman-CytoFLEX-Flow-Cytometer-300x256.jpg\" alt=\"\" width=\"300\" height=\"256\" srcset=\"https:\/\/research.tcu.edu.tw\/wp-content\/uploads\/2020\/12\/13_\u260513\u8272\u6d41\u5f0f\u7d30\u80de\u5100_Beckman-CytoFLEX-Flow-Cytometer-300x256.jpg 300w, https:\/\/research.tcu.edu.tw\/wp-content\/uploads\/2020\/12\/13_\u260513\u8272\u6d41\u5f0f\u7d30\u80de\u5100_Beckman-CytoFLEX-Flow-Cytometer-435x371.jpg 435w, https:\/\/research.tcu.edu.tw\/wp-content\/uploads\/2020\/12\/13_\u260513\u8272\u6d41\u5f0f\u7d30\u80de\u5100_Beckman-CytoFLEX-Flow-Cytometer.jpg 692w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<\/div>\n<\/div><\/div><\/div><div id=\"pgc-6047-0-1\"  class=\"panel-grid-cell\" ><div id=\"panel-6047-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<ul>\n<li><a href=\"https:\/\/research.tcu.edu.tw\/wp-content\/uploads\/2021\/11\/\u6536\u8cbb\u5e8f\u865f05_\u4f7f\u7528\u898f\u7bc4_13\u8272\u6d41\u5f0f\u7d30\u80de\u5206\u6790\u5100Beckman-Coulter-CytoFLEX.pdf\" target=\"_blank\" rel=\"noopener\"><strong>\u5100\u5668\u4f7f\u7528\u898f\u7bc4 Terms of service<\/strong><\/a><\/li>\n<li><strong><a href=\"https:\/\/drive.google.com\/drive\/folders\/1oq5qGZDpVamZoGJ6TFQOQ2TRCZMqKIsh?usp=drive_link\" target=\"_blank\" rel=\"noopener\">\u64cd\u4f5c\u624b\u518a User Manual<\/a><\/strong><\/li>\n<li><strong><a href=\"https:\/\/www.youtube.com\/watch?v=5XUXag5yeQA&amp;list=PLTCn8VtksnPmJjM2ZLDXso854lJh6PIlR\" target=\"_blank\" rel=\"noopener\">\u6559\u80b2\u8a13\u7df4\u5f71\u7247 Video<\/a><\/strong><\/li>\n<li><strong><a href=\"https:\/\/research.tcu.edu.tw\/?page_id=2919\" target=\"_blank\" rel=\"noopener\">\u5100\u5668\u6536\u8cbb Fee Structure<\/a><\/strong><\/li>\n<li><strong><a href=\"https:\/\/cfcreservation.tcu.edu.tw\/\" target=\"_blank\" rel=\"noopener\">\u5100\u5668\u9810\u7d04 Booking system<\/a><\/strong><\/li>\n<li><strong>\u5100\u5668\u7ba1\u7406\u54e1\uff1a\u85cd\u6587\u8c6a \u6280\u8853\u54e1 #11409<\/strong><\/li>\n<li>\u7ba1\u7406\u54e1\u4fe1\u7bb1\uff1a<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-9632 size-full\" src=\"https:\/\/research.tcu.edu.tw\/wp-content\/uploads\/2022\/01\/\u6587\u8c6aEmail.jpg\" alt=\"\" width=\"261\" height=\"28\" \/><\/li>\n<li>\u4ee3\u7406\u5546\/Agent :\u00a0<a href=\"https:\/\/www.beckman.tw\/flow-cytometry\/research-flow-cytometers\/cytoflex\">\u8c9d\u514b\u66fc Beckman<\/a><\/li>\n<\/ul>\n<p><u><b>\u653e\u7f6e\u5730\u9ede<br \/>\n<\/b><\/u>\u6148\u6fdf\u5927\u5b78\u4e2d\u592e\u6821\u5340\u52e4\u8015\u6a13 D603-6\u00a0 \u6d41\u5f0f\u7d30\u80de\u5100\u5206\u6790\u5be6\u9a57\u5ba4<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><div id=\"pg-6047-1\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-6047-1-0\"  class=\"panel-grid-cell\" ><div id=\"panel-6047-1-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"2\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<p><u><b>\u5100\u5668<\/b><\/u><u><b>\u4ecb\u7d39<\/b><\/u><br \/>\n\u6d41\u5f0f\u7d30\u80de\u5100\u70ba\u5206\u6790\u5149\u6563\u5c04\u4fe1\u865f\u548c\u87a2\u5149\u4fe1\u865f\uff0c\u5229\u7528\u5149\u8a0a\u865f\u7684\u5f37\u5f31\u548c\u6ce2\u9577\u4e0d\u540c\uff0c\u80fd\u5920\u5f97\u77e5\u7d30\u80de\u5927\u5c0f\u3001\u578b\u614b\u8207\u9846\u7c92\u5316\uff0c\u63d0\u4f9b\u591a\u7a2e\u7d30\u80de\u751f\u7269\u5b78\u7279\u6027\uff0c\u4ee5\u61c9\u7528\u65bc\u7d30\u80de\u5be6\u9a57\u4e4b\u4e2d\u3002CytoFLEX Flow Cytometer \u5c07\u5149\u5b78\u90e8\u4ef6\u548c\u6d41\u52d5\u5ba4\u9032\u884c\u6574\u5408\uff0c \u4f7f\u5149\u5728\u50b3\u8f38\u4e2d\u7684\u640d\u5931\u964d\u5230\u6700\u4f4e\uff0c\u5f9e\u800c\u5e36\u4f86\u6700\u4f73\u7684\u5100\u5668\u9748\u654f\u5ea6\u3002\u672c\u5100\u5668\u6700\u9ad8\u53ef\u4ee5\u4f7f\u7528\u5341\u4e09\u8272\u96f7\u5c04\u5149\u8b5c\uff0c\u76f8\u5bb9\u5404\u985e\u87a2\u5149\u67d3\u5291\u642d\u914d\uff0c\u53ef\u81ea\u7531\u904b\u7528\u642d\u914d\u3002\u53ef\u9760\u7684\u63a1\u6a23\u548c\u6d41\u9ad4\u7cfb\u7d71\u8a2d\u8a08\u652f\u63f4\u5fae\u91cf\u4e0a\u6a23\u7ba1\u7684\u6a23\u672c\u7372\u53d6\uff0c \u6a23\u672c\u4e0a\u6a23\u9ad4\u7a4d\u6700\u5c0f\u9054\u523010 ul\u3002CytoFLEX\u5728\u591a\u53c3\u6578\u61c9\u7528\u4e2d\u540c\u6a23\u5c55\u73fe\u51fa\u5f37\u5927\u5be6\u529b\uff0c\u5c0d\u5fae\u9846\u7c92\u7684\u6aa2\u6e2c\u6975\u9650\u53ef\u4f4e\u81f3200 nm\u3002\u6d41\u5f0f\u7d30\u80de\u5100\u6700\u4e3b\u8981\u7684\u5be6\u9a57\u5206\u6790\u6709\u7d30\u80de\u9031\u671f\u5206\u6790\u3001\u7d30\u80de\u51cb\u4ea1\u3001\u7d30\u80de\u578b\u614b\u3001\u7d30\u80de\u5167pH\u503c\u6e2c\u5b9a\u3001\u7d30\u80de\u5167\u9223\u96e2\u5b50\u6fc3\u5ea6\u6e2c\u5b9a\uff0c\u53ef\u61c9\u7528\u65bc\u816b\u7624\u5b78\u3001\u514d\u75ab\u5b78\u3001\u8840\u6db2\u5b78\u548c\u85e5\u7406\u5b78\u4e4b\u61c9\u7528\u7814\u7a76\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>A <strong>Flow Cytometer<\/strong> analyzes light scatter and fluorescent signals. By utilizing the differing intensities and wavelengths of these optical signals, it can determine various cell biological characteristics such as <strong>cell size, morphology, and granularity<\/strong>, which are applied in cell experiments.<\/p>\n<p>The <strong>CytoFLEX Flow Cytometer<\/strong> integrates optical components and the flow cell to minimize light transmission loss, thereby delivering optimal instrument sensitivity.<\/p>\n<p>This instrument can use up to <strong>13-color laser spectrums<\/strong>, compatible with various fluorescent dyes for flexible configuration. Its reliable sampling and fluidic system design supports <strong>micro-volume sample acquisition<\/strong>, with a minimum sample volume of <strong>10 \u00b5l<\/strong>.<\/p>\n<p>The CytoFLEX also demonstrates strong capability in multi-parameter applications, with a detection limit for <strong>microparticles<\/strong> as low as <strong>200 nm<\/strong>.<\/p>\n<p>The main experimental analyses performed by a flow cytometer include <strong>cell cycle analysis, apoptosis, cell morphology, intracellular pH measurement, and intracellular calcium ion concentration measurement<\/strong>. It is applicable in research areas such as <strong>oncology, immunology, hematology, and pharmacology<\/strong>.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><div id=\"pg-6047-2\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-6047-2-0\"  class=\"panel-grid-cell\" ><div id=\"panel-6047-2-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"3\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<p><a href=\"http:\/\/research.tcu.edu.tw\/?page_id=6877\" target=\"_blank\" rel=\"noopener\"><u><b>\u6210\u679c\u767c\u8868<\/b><\/u><\/a><\/p>\n<ol>\n<li><strong>Jaceosidin, a natural polymethoxyflavone, suppresses leukemia cell growth and impairs lipid metabolism-dependent survival in AML and cytarabine-resistant cells<\/strong><br \/>\nTrung Q. Phan, Je-Wen Liou, Liang-In Lin, Wei-Han Huang, Ming-Jiuan Wu, Pei-Yi Chen, Jui-Hung Yen<br \/>\nBiomed Pharmacother. 2025 Nov:192:118654. <a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0753332225008480?via%3Dihub\" target=\"_blank\" rel=\"noopener\">doi: 10.1016\/j.biopha.2025.118654.<\/a><br \/>\n-<\/li>\n<li><strong>Therapeutic Potential of Salvia miltiorrhiza Root Extract in Alleviating Cold-Induced Immunosuppression<\/strong><br \/>\nChi-Cheng Li ,Song-Lin Liu ,Te-Sheng Lien ,Der-Shan Sun ,Ching-Feng Cheng ,Hussana Hamid ,Hao-Ping Chen ,Tsung-Jung Ho ,I-Hsin Lin ,Wen-Sheng Wu ,Chi-Tan Hu ,Kuo-Wang Tsai and Hsin-Hou Chang<br \/>\nInt. J. Mol. Sci. 2024, 25(17), 9432;<a href=\"https:\/\/www.mdpi.com\/1422-0067\/25\/17\/9432\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.3390\/ijms25179432<\/a><br \/>\n-<\/li>\n<li><strong>Targeted Delivery to Dying Cells Through P-Selectin\u2013PSGL-1 Axis: A Promising Strategy for Enhanced Drug Efficacy in Liver Injury Models<\/strong><br \/>\nTe-Sheng Lien ,Der-Shan Sun and Hsin-Hou Chang<br \/>\nCells 2024, 13(21), 1778;<a href=\"https:\/\/www.mdpi.com\/2073-4409\/13\/21\/1778\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.3390\/cells13211778<\/a><br \/>\n-<\/li>\n<li><strong>Dengue Envelope Protein as a Cytotoxic Factor Inducing Hemorrhage and Endothelial Cell Death in Mice<\/strong><br \/>\nTe-Sheng Lien ,Der-Shan Sun ,Wen-Sheng Wu and Hsin-Hou Chang<br \/>\nInt. J. Mol. Sci. 2024, 25(19), 10858;<a href=\"https:\/\/www.mdpi.com\/1422-0067\/25\/19\/10858\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.3390\/ijms251910858<\/a><br \/>\n-<\/li>\n<li><strong>Restraint Stress-Induced Neutrophil Inflammation Contributes to Concurrent Gastrointestinal Injury in Mice<\/strong><br \/>\nRina Munalisa ,Te-Sheng Lien ,Ping-Yeh Tsai ,Der-Shan Sun ,Ching-Feng Cheng ,Wen-Sheng Wu ,Chi-Cheng Li ,Chi-Tan Hu ,Kuo-Wang Tsai ,Yungling Leo Lee ,Yu-Chi Chou and Hsin-Hou Chang<br \/>\nInt. J. Mol. Sci. 2024, 25(10), 5261;<a href=\"https:\/\/www.mdpi.com\/1422-0067\/25\/10\/5261\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.3390\/ijms25105261<\/a><br \/>\n-<\/li>\n<\/ol>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>\u5100\u5668\u4f7f\u7528\u898f\u7bc4 Terms of service \u64cd\u4f5c\u624b\u518a U [&hellip;]<\/p>\n","protected":false},"author":12,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-6047","page","type-page","status-publish","hentry","clearfix"],"_links":{"self":[{"href":"https:\/\/research.tcu.edu.tw\/index.php?rest_route=\/wp\/v2\/pages\/6047","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/research.tcu.edu.tw\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/research.tcu.edu.tw\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/research.tcu.edu.tw\/index.php?rest_route=\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"https:\/\/research.tcu.edu.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6047"}],"version-history":[{"count":16,"href":"https:\/\/research.tcu.edu.tw\/index.php?rest_route=\/wp\/v2\/pages\/6047\/revisions"}],"predecessor-version":[{"id":15672,"href":"https:\/\/research.tcu.edu.tw\/index.php?rest_route=\/wp\/v2\/pages\/6047\/revisions\/15672"}],"wp:attachment":[{"href":"https:\/\/research.tcu.edu.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6047"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}