正立螢光顯微鏡_Nikon Ni-E

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• 儀器管理員：林靜慧 技術員 #11409
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放置地點
慈濟大學中央校區勤耕樓 D329  生物影像實驗室

儀器介紹

• 雙層平行光路設計；全新的雙層平行光路設計，打破以往的單層光路，使Ni系列可以同時加裝CCD影像裝置和高階系統組件如雙層螢光濾片轉盤、Photoactivation module、multiphoton detector (NDD)，使得可應用的層面更加靈活與寬廣。
• 雙光子影像擷取，提供更深層的影像資料，透過與Nikon A1R Multiphoton 系統的組合，及高感度的NDD(non-descanned detector)，使得Ni可以擷取樣品更深層以及更清晰的影像，另外透過改變對焦系統由載物台變成鼻輪調整焦距，使載物台可以固定位置，可以方便進行實驗。

 

Dual-Layer Parallel Light Path Design: The brand-new dual-layer parallel light path design breaks away from the previous single-layer light path, allowing the Ni series to simultaneously install a CCD imaging device and high-end system components such as a dual-layer fluorescence filter turret, Photoactivation module, and Multiphoton Detector (NDD), making the range of applications more flexible and broad.

Two-Photon Image Acquisition: Provides deeper image data. Through combination with the Nikon A1R Multiphoton system and the highly sensitive non-descanned detector (NDD), the Ni can capture deeper and clearer images of the sample. Furthermore, by changing the focusing system from a stage-based mechanism to nosepiece-based focus adjustment, the stage can remain fixed, which facilitates experiments.

Instrument Specifications
• Objectives: 10X, 20X, 40X, 60X (Oil), 100X (Oil)
• Reflectors (Filter Sets): DAPI, FITC, TRITC, Cy5

成果發表

1. Tanshinone IIA Downregulates Lipogenic Gene Expression and Attenuates Lipid Accumulation through the Modulation of LxRα/SREBP1 Pathway in HepG2 Cells
   Wan-Yun Gao, Pei-Yi Chen, Hao-Jen Hsu, Ching-Yen Lin, Ming-Jiuan Wu, Jui-Hung Yen
   Biomedicines; 2021 Mar 23;9(3):326. doi: 10.3390/biomedicines9030326.
   
   

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2. 4-(Phenylsulfanyl) Butan-2-One Attenuates the Inflammatory Response Induced by Amyloid-β Oligomers in Retinal Pigment Epithelium Cells.
   Peeraporn Varinthra, Shun-Ping Huang, Supin Chompoopong, Zhi-Hong Wen, Ingrid Y Liu
   Marine Drugs ; 2020 Dec 23;19(1):1. doi: 10.3390/md19010001